Deleted
Sample Discontinued
Experience: None
Not Used
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DNA
CBPa-BpA

Part:BBa_K2053001:Design

Designed by: Mathieu Hubert   Group: iGEM16_Pasteur_Paris   (2016-10-11)


Fusion cellulose-binding domain of CBP-B domain of protein A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 79
    Illegal AgeI site found at 379
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Polyhistidine tag (6 His) sequence was added at the 5'end as well as pET43.1a-derived Lac operator, ribosome-binding site (RBS), and T7 terminator.


Source

Cellulose-binding domain of cellulose-binding protein A was taken from iGEM Bielefeld-Germany 2012 team (BBa_K863110) and B domain of protein A was taken from iGEM Warsaw 2008 (BBa_K103003).

References